Original description as Gambusia longispinis:
- Minckley, Wendell L. 1962. "Two new species of fishes of the genus Gambusia (Poeciliidae) from northeastern Mexico". Copeia. 162(2):391-396 (ffm04578)
Conservation: Gambusia longispinis is not evaluated by the international union for the conservation of nature in the iucn red list of threatened species.